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- Title
AFP 蛋白芯片技术的检测流程优化.
- Authors
刘海东; 王振顺; 刘超; 张爱英; 李宁
- Abstract
Objective: To optimize the traditional protein microarray procedure for detecting AFP (Alpha Fetal protein) by short- ening the detection time, providing an effective, economical, easily performed and time-saving method for screening primary liver cancer. Methods: The optimized procedure significantly shortened the detection time to 18 minutes on the basis of traditional protein microarray detection procedure (1-1.5 hours). Serum sample was examined using improved and traditional procedure of protein microarray to verify the accuracy of improved procedure. Results: Compared with the traditional procedure of protein mieroarray, the improved procedure shortened the detection time to 18minutes. The results of repetition test of the same sample indicated that no statistically significant dif- ference was found in the AFP levels between traditional procedure and improved procedure (16.50± 1.172 ng/mL vs 18.33 ± 1.029 ng/mL, p=0.251). Conclusion: On the premise of ensuring detection accuracy, this study successfully optimized the AFP protein mieroar- ray detection procedure by shortening the detection time.
- Publication
Progress in Modern Biomedicine, 2018, Vol 18, Issue 1, p146
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2018.01.032