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- Title
Evaluating the Mechanism of Cell Death in Melanoma Induced by the Cannabis Extract PHEC-66.
- Authors
Bachari, Ava; Nassar, Nazim; Telukutla, Srinivasareddy; Zomer, Roby; Piva, Terrence J.; Mantri, Nitin
- Abstract
Research suggests the potential of using cannabinoid-derived compounds to function as anticancer agents against melanoma cells. Our recent study highlighted the remarkable in vitro anticancer effects of PHEC-66, an extract from Cannabis sativa, on the MM418-C1, MM329, and MM96L melanoma cell lines. However, the complete molecular mechanism behind this action remains to be elucidated. This study aims to unravel how PHEC-66 brings about its antiproliferative impact on these cell lines, utilising diverse techniques such as real-time polymerase chain reaction (qPCR), assays to assess the inhibition of CB1 and CB2 receptors, measurement of reactive oxygen species (ROS), apoptosis assays, and fluorescence-activated cell sorting (FACS) for apoptosis and cell cycle analysis. The outcomes obtained from this study suggest that PHEC-66 triggers apoptosis in these melanoma cell lines by increasing the expression of pro-apoptotic markers (BAX mRNA) while concurrently reducing the expression of anti-apoptotic markers (Bcl-2 mRNA). Additionally, PHEC-66 induces DNA fragmentation, halting cell progression at the G1 cell cycle checkpoint and substantially elevating intracellular ROS levels. These findings imply that PHEC-66 might have potential as an adjuvant therapy in the treatment of malignant melanoma. However, it is essential to conduct further preclinical investigations to delve deeper into its potential and efficacy.
- Subjects
CELL death; CANNABINOID receptors; CELL cycle; GENE expression; CELL analysis; REACTIVE oxygen species; CANNABIS (Genus); CANNABINOIDS
- Publication
Cells (2073-4409), 2024, Vol 13, Issue 3, p268
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells13030268