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- Title
Impact of Microbiota Depletion by Antibiotics on SARS-CoV-2 Infection of K18-hACE2 Mice.
- Authors
Rodrigues, Patrícia Brito; Gomes, Giovanni Freitas; Angelim, Monara K. S. C.; Souza, Gabriela F.; Muraro, Stefanie Primon; Toledo-Teixeira, Daniel A.; Rattis, Bruna Amanda Cruz; Passos, Amanda Stephane; Pral, Laís Passarielo; de Rezende Rodovalho, Vinícius; dos Santos P. Gomes, Arilson Bernardo; Matheus, Valquíria Aparecida; Antunes, André Saraiva Leão Marcelo; Crunfli, Fernanda; Antunes, Krist Helen; de Souza, Ana Paula Duarte; Consonni, Sílvio Roberto; Leiria, Luiz Osório; Alves-Filho, José Carlos; Cunha, Thiago M.
- Abstract
Clinical and experimental data indicate that severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infection is associated with significant changes in the composition and function of intestinal microbiota. However, the relevance of these effects for SARS-CoV-2 pathophysiology is unknown. In this study, we analyzed the impact of microbiota depletion after antibiotic treatment on the clinical and immunological responses of K18-hACE2 mice to SARS-CoV-2 infection. Mice were treated with a combination of antibiotics (kanamycin, gentamicin, metronidazole, vancomycin, and colistin, Abx) for 3 days, and 24 h later, they were infected with SARS-CoV-2 B lineage. Here, we show that more than 80% of mice succumbed to infection by day 11 post-infection. Treatment with Abx had no impact on mortality. However, Abx-treated mice presented better clinical symptoms, with similar weight loss between infected–treated and non-treated groups. We observed no differences in lung and colon histopathological scores or lung, colon, heart, brain and kidney viral load between groups on day 5 of infection. Despite some minor differences in the expression of antiviral and inflammatory markers in the lungs and colon, no robust change was observed in Abx-treated mice. Together, these findings indicate that microbiota depletion has no impact on SARS-CoV-2 infection in mice.
- Subjects
ANTIBIOTICS; SARS-CoV-2; GUT microbiome; MICE; COLISTIN
- Publication
Cells (2073-4409), 2022, Vol 11, Issue 16, p2572
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells11162572