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- Title
Polysaccharides from Vaccaria segetalis seeds reduce urinary tract infections by inhibiting the adhesion and invasion abilities of uropathogenic Escherichia coli.
- Authors
Rongmei Yao; Xin Mao; Yingli Xu; Xue Qiu; Lirun Zhou; Yaxin Wang; Bo Pang; Mengping Chen; Shan Cao; Lei Bao; Yanyan Bao; Shanshan Guo; Limin Hu; Haijiang Zhang; Xiaolan Cui
- Abstract
The seeds of Vaccaria segetalis (Neck.) are from a traditional medicinal plant Garcke, also called Wang-Bu-Liu-Xing in China. According to the Chinese Pharmacopoeia, the seeds of V. segetalis can be used for treating urinary system diseases. This study was designed to investigate the underlying mechanism of VSP (polysaccharides from Vaccaria segetalis) against urinary tract infections caused by uropathogenic Escherichia coli (UPEC). Here, both in vitro and in vivo infection models were established with the UPEC strain CFT073. Bacterial adhesion and invasion into bladder epithelial cells were analyzed. We found that VSP reduced the adhesion of UPEC to the host by inhibiting the expression of bacterial hair follicle adhesion genes. VSP also reduced the invasion of UPEC by regulating the uroplakins and Toll-like receptors of host epithelial cells. In addition, the swarming motility and flagella-mediated motility genes flhC, flhD and Flic of UPEC were diminished after VSP intervention. Taken together, our findings reveal a possible mechanism by which VSP interferes with the adhesion and invasion of UPEC.
- Subjects
CHINA; URINARY tract infections; ESCHERICHIA coli; POLYSACCHARIDES; HAIR follicles; BACTERIAL adhesion
- Publication
Frontiers in Cellular & Infection Microbiology, 2022, Vol 12, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2022.1004751