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- Title
Identification of radiation-specific responses from gene expression profile.
- Authors
Park, Woong-Yang; Hwang, Chang-Il; Im, Chang-Nim; Kang, Min-Ji; Woo, Jang-Hee; Kim, Ju-Hoon; Kim, Yon Su; Kim, Ju-Han; Kim, Ho; Kim, Kyung-A; Yu, Hyung-Jin; Lee, Sue-Jae; Lee, Yun-Sil; Seo, Jeong-Sun
- Abstract
The responses to ionizing radiation (IR) in tumors are dependent on cellular context. We investigated radiation-related expression patterns in Jurkat T cells with nonsense mutation in p53 using cDNA microarray. Expression of 2400 genes in γ-irradiated cells was distinct from other stimulations like anti-CD3, phetohemagglutinin (PHA) and concanavalin A (ConA) in unsupervised clustering analysis. Among them, 384 genes were selected for their IR-specific changes to make ‘RadChip’. In spite of p53 status, every type of cells showed similar patterns in expression of these genes upon γ-radiation. Moreover, radiation-induced responses were clearly separated from the responses to other genotoxic stress like UV radiation, cisplatin and doxorubicin. We focused on two IR-related genes, phospholipase Cγ2 (PLCG2) and cytosolic epoxide hydrolase (EPHX2), which were increased at 12 h after γ-radiation in RT–PCR. TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-κB upon γ-radiation. From these results, we could identify the IR-specific genes from expression profiling, which can be used as radiation biomarkers to screen radiation exposure as well as probing the mechanism of cellular responses to ionizing radiation.Oncogene (2002) 21, 8521–8528. doi:10.1038/sj.onc.1205977
- Subjects
GENE expression; TUMORS; DOXORUBICIN; BIOMARKERS
- Publication
Oncogene, 2002, Vol 21, Issue 55, p8521
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1205977