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- Title
Multinucleated Retinal Pigment Epithelial Cells Adapt to Vision and Exhibit Increased DNA Damage Response.
- Authors
Ke, Qin; Gong, Lili; Zhu, Xingfei; Qi, Ruili; Zou, Ming; Chen, Baoxin; Liu, Wei; Huang, Shan; Liu, Yizhi; Li, David Wan-Cheng
- Abstract
Multinucleated retinal pigment epithelium (RPE) cells have been reported in humans and other mammals. Rodents have an extremely high percentage of multinucleated cells (more than 80%). Both mouse and human multinucleated RPE cells exhibit specific regional distributions that are potentially correlated with photoreceptor density. However, detailed investigations of multinucleated RPE in different species and their behavior after DNA damage are missing. Here, we compared the composition of multinucleated RPE cells in nocturnal and diurnal animals that possess distinct rod and cone proportions. We further investigated the reactive oxygen species (ROS) production and DNA damage response in mouse mononucleated and multinucleated RPE cells and determined the effect of p53 dosage on the DNA damage response in these cells. Our results revealed an unrealized association between multinucleated RPE cells and nocturnal vision. In addition, we found multinucleated RPE cells exhibited increased ROS production and DNA damage after X-ray irradiation. Furthermore, haploinsufficiency of p53 led to increased DNA damage frequency after irradiation, and mononucleated RPE cells were more sensitive to a change in p53 dosage. In conclusion, this study provides novel information on in vivo PRE topography and the DNA damage response, which may reflect specific requirements for vision adaption and macular function.
- Subjects
DNA damage; RHODOPSIN; CHROMATOPHORES; EPITHELIAL cells; PHOTORECEPTORS; REACTIVE oxygen species; NOCTURNAL animals
- Publication
Cells (2073-4409), 2022, Vol 11, Issue 9, pN.PAG
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells11091552