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- Title
A quick, easy and cost-effective in planta method to develop direct transformants in wheat.
- Authors
Tarafdar, Avijit; Vishwakarma, Harinder; Gothandapani, S.; Bhati, Meenal; Biswas, Koushik; Prakash, Arul; Chaturvedi, Uttara; Solanke, Amolkumar U.; Padaria, Jasdeep Chatrath
- Abstract
Agrobacterium mediated in planta method was used to transform Indian elite wheat genotype HD2894 with herbicide-tolerant CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene. The apical meristems of germinated seeds were targeted for introgression of transgene. The obtained T1 plants were screened by spraying 1% glyphosate and only positive transformants survived. The presence of transgene was also confirmed by PCR and Southern hybridization. Using this method, 3.07% transformation rate was observed. To identify transgenic lines carrying stably integrated CP4-EPSPS gene, the transgenic populations were screened in T3 generation using 1% glyphosate and lines with 100% survival were considered as homozygous. No significant morpho-physiological variations were observed within the transgenic lines as compared to non-transgenic plants. The present study resulted in herbicide-tolerant transgenic wheat and provides a valuable tool for development of wheat genetic transformation.
- Subjects
WHEAT; GENETIC transformation; GLYPHOSATE; AGROBACTERIUM tumefaciens; MERISTEMS; AGROBACTERIUM
- Publication
3 Biotech, 2019, Vol 9, Issue 5, pN.PAG
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-019-1708-6