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- Title
Genome re-diploidization occurs spontaneously just prior to anthesis in artificially induced auto-tetraploid maize (Zea mays L.) inbred lines.
- Authors
Ahmadi, Behzad; Ebrahimzadeh, Hamed; Zarifi, Eissa
- Abstract
In this study, various concentrations of oryzalin (0, 5, 10, 50, and 100 mg l−1), trifluralin (0, 5, 10, 50, and 100 mg l−1), and colchicine (0, 250, 500, 1000, and 5000 mg l−1) each for 12, 24, or 48 h were tested on auto-tetraploid production in Zea mays L. inbred lines "S61" and "K1263/17". The highest rates of tetraploids were obtained in the inbred line "S61" following 50 mg l−1 oryzalin for 24 h (11.1%) and colchicine treatment at 1000 mg l−1 for 24 h (12.2%) and 48 h (15.8%). In the inbred line "K1263/17", tetraploids were detected with trifluralin at 10 mg l−1 (2.3%) and 50 mg l−1 (6.9%) both for 24 h. High rate of tetraploid production (19.5%) was also observed in the presence of 1000 mg l−1 colchicine for 24 h. Compared to their diploid counterparts, the regenerated tetraploids exhibited relatively lower survival rate, and only 33.3% of the inbred line "K1263/17"-derived tetraploids survived and developed. Moreover, all survived regenerants kept their altered ploidy level until the seventh-leaf stage, and then turned the ploidy level back to the diploid state prior to the anthesis. Except for plant height, basal stem diameter, and total chlorophyll content of the seventh leaves, the diploid and re-diploidized tetraploids were not significantly different in terms of other morphological features assessed. Although it was possible to induce polyploidy in Z. mays L., the auto-tetraploids re-diploidized their genome simultaneously before anthesis, offering a precious platform to study the mechanism of genome downsizing in greater detail in this species. Key message: Auto-tetraploids can be induced in Zea mays L. using colchicine and dinitroaniline herbicides. The generated tetraploids either die or downsize their genome prior to anthesis.
- Subjects
SURVIVAL rate; CORN; GENOMES; POLYPLOIDY; PLOIDY; HERBICIDES; TRIFLURALIN
- Publication
Plant Cell, Tissue & Organ Culture, 2021, Vol 146, Issue 1, p115
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-021-02051-0