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- Title
Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation.
- Authors
Ayon Haro, E. R.; Ukai, T.; Yokoyama, M.; Kishimoto, T.; Yoshinaga, Y.; Hara, Y.
- Abstract
Ayon Haro ER, Ukai T, Yokoyama M, Kishimoto T, Yoshinaga Y, Hara Y. Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation. J Periodont Res 2011; 46: 361-373. © 2011 John Wiley & Sons A/S Interferon-γ (IFN-γ) potently inhibits RANKL-induced osteoclastogenesis in vitro. In contrast, previous studies have shown that an increase in IFN-γ expression is correlated with an increase in lipopolysaccharide (LPS)-induced bone loss in vivo. However, it is not clear whether local IFN-γ accelerates osteoclastogenesis or not in vivo. Therefore, the aim of this study was to clarify the role of local IFN-γ in LPS-induced osteoclastogenesis. We induced bone loss in calvaria by injecting LPS. One group of mice received an IFN-γ injection together with LPS injection, while another group received IFN-γ 2 d after LPS injection. Bone resorption was observed histologically. Next, we stimulated murine bone marrow macrophages with macrophage-colony stimulating factor and RANKL in vitro. We added different doses of IFN-γ and/or LPS at 0 or 48 h time points. Cells were stained with tartrate-resistant acid phosphatase at 72 h. Local administration of IFN-γ together with LPS injection did not affect osteoclast formation. However, IFN-γ injected after LPS injection accelerated osteoclast formation. Also, we confirmed that IFN-γ added at 0 h inhibited RANKL-induced osteoclastogenesis in vitro. However, inhibition by IFN-γ added at 48 h was reduced compared with that by IFN-γ added at 0 h. Interestingly, IFN-γ together with a low concentration of LPS accelerated osteoclast formation when both were added at 48 h compared with no addition of IFN-γ. The results suggest that local IFN-γ accelerates osteoclastogenesis in certain conditions of LPS-induced inflammatory bone loss.
- Subjects
ANALYSIS of variance; ANIMAL experimentation; BIOPHYSICS; BONE growth; COMPUTER software; FLOW cytometry; IMMUNOHISTOCHEMISTRY; INJECTIONS; INTERFERONS; RESEARCH methodology; MICE; INDUSTRIAL research; DATA analysis; STATISTICAL significance
- Publication
Journal of Periodontal Research, 2011, Vol 46, Issue 3, p361
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.2011.01352.x