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- Title
P202 - NOVEL BIOMARKERS OF RENAL SCAR FORMATION IN ACUTE PYELONEPHRITIS IN RATS.
- Authors
GÜLCAN KURT, Yasemin; ÇALIŞKAN, Bahadır; GÜVEN, Ahmet; AKGÜL, Emin Özgür; UYSAL, Bülent; ALTAN, Bilal; ÖZCAN, Ayhan; BEDİR, Orhan; SÜRER, İhami; KORKMAZ, Ahmet
- Abstract
Aim: This time-course study was designed to determine the effect of progression of acute pyelonephritis (APN) on oxidative/nitrossative stress parameters, serum procalcitonin and neopterin levels, and to correlate them with renal structure. Materials and Methods: Fifty rats were divide ito five gorups often rats each. Eight rats in each gorup underwent APN procedure. the other two rats in each group served as a sham-operated control gorup. APN was induced by injection of 0.1 mL freshly prepared E. coli solution into the both kidneys. the rats in each gorup were evaluated after 1,3,7,14 and 4 days of APN procedure, respectively. At the end of the experimental period, animals were killed via decapitation and trunk blood was collected. Both kidneys were harvested for antioxidant enzyme activity (superoxide dismutase (SOD) and glutathione peroxidase (GPx)), oxidative stress parameters (tissue lipid peroxidation (MDA) and protein carbonyl content (PCC), nitrosative stress (NOx), hidroxyprolin (HP) level and histopatholojic examination. Nitrate plus nitrite (NOx) concentrations were measured by using ion chromatography, serum neopterin (NP) levels were determined by using a High Pressure Liquid Chromatography (HPLC) system with a fluorescence detector, serum procalcitonin (PCT) levels were measured by automated immunofluroscent assay. Results: Both oxidative/nitrosative stress parameters and antioxidant enzyme activities increased progressively, but after 14th day to control values. Tissue HP level was increased progressively througout the experiemtnal period suggesting increased scar formation which confirmed with histopathologic evaluation. Histopathologic evaluation also showed that injury score showed a correlation with biochemical evaluation. Conclusion: This study showed that there is a clear correlation between oxidative/nitrossative stress parameters and renal scar formation in APN. Serum NP and PCT levels antioxidative stress parameters in acute period can be used to determination of renal scar formation in chronic period.
- Subjects
BIOMARKERS; BIOCHEMISTRY; PYELONEPHRITIS; KIDNEY diseases; LABORATORY rats
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2012, Vol 37, Issue S1, p343
- ISSN
0250-4685
- Publication type
Article