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- Title
Highly polymorphic microsatellite loci for the Gran Canarian skink ( Chalcides sexlineatus) and their applicability in other Canarian Chalcides.
- Authors
SUÁREZ, N. M.; BLOOR, P.; BROWN, R. P.; PESTANO, J.
- Abstract
The scincid lizard Chalcides sexlineatus is endemic to the island of Gran Canaria, within which it shows a pronounced phylogeographical pattern. Understanding the maintenance of this pattern requires knowledge of nuclear DNA structuring. Eleven polymorphic tetranucleotide microsatellite loci were isolated from a library constructed from genomic DNA enriched for AAAG repeat sequence. The number of alleles per locus ranged from 12 to 19, and expected heterozygosities spanned from 0.738 to 0.933 ( n = 30). The majority of these could be cross-amplified in other endemic Canarian Chalcides. The loci will be used to investigate gene flow between the principal mitochondrial clades within C. sexlineatus.
- Subjects
CANARY Islands; LIZARDS; DNA; CHALCIDES; MITOCHONDRIAL DNA; MICROSATELLITE repeats; GRAN Canaria (Canary Islands)
- Publication
Molecular Ecology Resources, 2008, Vol 8, Issue 3, p666
- ISSN
1755-098X
- Publication type
Article
- DOI
10.1111/j.1471-8286.2007.02040.x