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- Title
Platelet-rich plasma improves lipopolysaccharide-induced inflammatory response by upgrading autophagy.
- Authors
Li, Yanhui; Shao, Chunyan; Zhou, Mou; Shi, Linying
- Abstract
Objectives: Platelet-rich plasma (PRP) plays an important role at all stages of wound healing, including the inflammatory stage. Macrophage autophagy has been found to influence the inflammatory response process. However, it is unclear whether PRP can affect inflammatory responses via macrophage autophagy. In the present study, we explored the effect of PRP on inflammatory responses and researched the underlying mechanism. Methods: RAW 264.7 macrophages were treated with PRP and/or lipopolysaccharide (LPS). The effects of PRP on the expression of inflammatory factors were determined by ELISA and qRT-PCR. Macrophage autophagosomes were also assessed by TEM and immunofluorescence. Autophagy and NLRP3-related proteins were investigated using Western blot analysis. Results: PRP reduced the levels of inflammatory factors and increased autophagy in RAW 264.7 cells. Pretreatment with 3-MA, which is an autophagy inhibitor, abolished the impact of PRP on the inflammatory response. Moreover, PRP induced macrophage autophagy by activating the NLRP3 inflammasome. Conclusions: These results show that PRP can attenuate LPS-induced inflammatory responses by enhancing autophagy via NLRP3. These study also provides a new perspective on the molecular mechanism of PRP therapy in wound healing.
- Subjects
PLATELET-rich plasma; INFLAMMATION; AUTOPHAGY; WESTERN immunoblotting; WOUND healing
- Publication
European Journal of Inflammation, 2022, p1
- ISSN
1721-727X
- Publication type
Article
- DOI
10.1177/1721727X221112271