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- Title
Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish
- Authors
Hu, Sheng-Nan; Yu, Hui; Zhang, Yun-Bin; Wu, Zhi-Li; Yan, Yuan-Chang; Li, Yi-Xue; Li, Yuan-Yuan; Li, Yi-Ping
- Abstract
Abstract: Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microarray analysis of mRNAs collected at the dome stage revealed that the Sb MO impairs activation of a large set of zygotic genes and reduces degradation of maternal mRNA during MBT. Furthermore, an apoptotic response occurs in Sb morphants at about 6hpf. SoxB1 family genes including sox31 thus play an essential role for early embryos traversing the transitional stage.
- Subjects
ZEBRA danio embryos; APOPTOSIS; MIDBLASTULA transition; RNA splicing; MICROARRAY technology; EMBRYOLOGY; BIOLOGICAL variation
- Publication
FEBS Letters, 2012, Vol 586, Issue 3, p222
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.12.008