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- Title
Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour.
- Authors
Albrecht, M.; Frungieri, M. B.; Kunz, L.; Rämsch, R.; Meineke, V.; Köhn, F. M.; Mayerhofer, A.
- Abstract
Fibroblast proliferation is a key process in tissue remodeling and mast cells (MCs) are thought to play a crucial role. Having established that the three major MC products, tryptase, histamine and TNF-alpha (TNF) are normally present in human skin MCs, which are in close proximity to dermal fibroblasts, we studied their individual effects on cell cycle-controlled human dermal fibroblasts (HFFF2). These cells express receptors (H1, PAR2, TNFR1/2) for the major MC mediators, but only tryptase or a PAR2 agonist peptide stimulated proliferation and gene expression. TNF was antimitotic, and histamine, while elevating intracellular Ca2+ levels at high concentrations, did not affect proliferation. We conclude that MC products but also composition and numbers of respective receptors on fibroblasts are crucially responsible for fibroproliferative events.
- Subjects
MAST cells; CONNECTIVE tissue cells; HISTAMINE; TUMOR necrosis factors; TISSUE remodeling; CALCIUM
- Publication
Cellular & Molecular Life Sciences, 2005, Vol 62, Issue 23, p2867
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-005-5289-7