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- Title
The Cav3.1 T-type calcium channel is required for neointimal formation in response to vascular injury in mice.
- Authors
Tzeng, Bing-Hsiean; Chen, Yen-Hui; Huang, Ching-Hui; Lin, Shin-Shiou; Lee, Kuan-Rong; Chen, Chien-Chang
- Abstract
Aims Restenosis is an undesirable consequence following percutaneous vascular interventions. However, the current strategy for preventing restenosis is inadequate. The aim of this study was to investigate the role of low-voltage gated T-type calcium channels in regulating vascular smooth muscle cell (VSMC) proliferation during neointimal formation. Methods and results Wire injury of mice carotid arteries resulted in neointimal formation in the wild-type and Cav3.2−/− but not Cav3.1−/− mice, indicating a critical role of Cav3.1 in neointimal formation. In addition, we found a significant increase of Cav3.1 mRNA and protein in injured arteries. Cav3.1 knockout or knockdown (shCav3.1) reduced VSMC proliferation. Since T-channels are expressed predominantly in the G1 and S phases in VSMCs, we examined whether an abnormal G1/S transition was the cause of the reduced cell proliferation in shCav3.1 VSMCs. We found a disrupted expression of cyclin E in shCav3.1 VSMCs, and calmodulin agonist CALP1 partially rescued the defective cell proliferation. Furthermore, we demonstrated that infusion of NNC55-0396, a selective T-channel blocker, inhibited neointimal formation in wild-type mice. Conclusion Cav3.1 is required for VSMC proliferation during neointimal formation, and blocking of Cav3.1 may be beneficial for preventing restenosis.
- Subjects
CALCIUM channels; BLOOD vessels; WOUNDS &; injuries; CORONARY restenosis prevention; VASCULAR smooth muscle; MUSCLE cells; CELL proliferation; CALCIUM antagonists; LABORATORY mice
- Publication
Cardiovascular Research, 2012, Vol 96, Issue 3, p533
- ISSN
0008-6363
- Publication type
Article
- DOI
10.1093/cvr/cvs257