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- Title
A Brief Survey of Methods to Count Cells from Tissue Sections.
- Authors
Asari, Mohd Asnizam; Yusof, Nurul Aiman Mohd
- Abstract
Objective: There are several methods in which cells can be counted from histological tissue sections. These include 3-D serial reconstruction, empirical method, model-based method and design-based method. Our objective is to investigate the strengths and limitations of each method with the hope of guiding researchers in deciding the most appropriate cell counting method for their work. Design: A critical review of published articles in the literature. Methods: Peer-reviewed articles were identified through computerized searches using PubMed and Science Direct search engines. Results: Cell counting using 3-D serial reconstruction is unbiased, but extremely inefficient and tedious. Empirical method is more efficient than 3-D serial reconstruction but less efficient in comparison to model-based and design-based methods. Model- based counting method has a long history of usage but it is vulnerable to geometrical bias. Design-based method is less vulnerable to geometrical bias but it still has its own set of limitations and biases. Conclusion: Each counting method has its own set of strengths and limitations. The choice of method does not only depend on intrinsic features of each method, but also the need of a particular experiment, expertise of investigators involved and available equipment.
- Subjects
CELLS; MEDLINE; ONLINE information services; SURVEYS; TISSUES; SYSTEMATIC reviews
- Publication
International Medical Journal, 2016, Vol 23, Issue 4, p365
- ISSN
1341-2051
- Publication type
Article