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- Title
The GABRG2 Mutation, Q351X, Associated with Generalized Epilepsy with Febrile Seizures Plus, Has Both Loss of Function and Dominant-Negative Suppression.
- Authors
Jing-Qiong Kang; Wangzhen Shen; Macdonald, Robert L.
- Abstract
TheGABAA receptor γ2 subunit mutation, Q351X, associated with generalized epilepsy with febrile seizures plus (GEFS+), created a loss of function with homozygous expression. However, heterozygous γ2(+/-) gene deletion mice are seizure free, suggesting that the loss of oneGABRG2allele alone in heterozygous patientsmaynot be sufficient to produce epilepsy. Hereweshow that the mutant γ2 subunit was immature and retained in the endoplasmic reticulum (ER). With heterozygous coexpression of γ2S/γ2S(Q351X) subunits andα 1 and β2 subunits, the trafficking deficient mutant γ2 subunit reduced trafficking of wild-type partnering subunits, which was not seen in the hemizygous gene deletion control. Consequently, the function of the heterozygous receptor channel was reduced to less than the hemizygous control and to less than half of the wild-type receptors with a full gene dose. Pulse-chase experiments demonstrated that in the presence of the mutant γ2S(Q351X) subunit, wild-type α1 subunits degraded more substantially within 1 h of translation. We showed that the basis for this dominant-negative effect on wild-type receptors was due to an interaction between mutant and wild-type subunits. The mutant subunit oligomerized with wild-type subunits and trapped them in the ER, subjecting them to glycosylation arrest and ER-associated degradation (ERAD) through the ubiquitin proteosome system. Thus, we hypothesize that a likely explanation for the GEFS+ phenotype is a dominant-negative suppression of wild-type receptors by the mutant γ2S subunit in combination with loss of mutant γ2S subunit protein function.
- Subjects
GENETIC mutation; EPILEPSY; DEVELOPMENTAL disabilities; FEBRILE seizures; GENETIC disorders; GLYCOSYLATION
- Publication
Journal of Neuroscience, 2009, Vol 29, Issue 9, p2845
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.4772-08.2009