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- Title
Spliced-Leader RNA as a Dynamic Marker for Monitoring Viable Leishmania Parasites During and After Treatment.
- Authors
Hendrickx, Rik; Melkamu, Roma; Tadesse, Dagimawie; Teferi, Tedla; Feijens, Pim-Bart; Vleminckx, Margot; Henten, Saskia van; Alves, Fabiana; Shibru, Tamiru; Griensven, Johan van; Caljon, Guy; Pareyn, Myrthe
- Abstract
Accurate detection of viable Leishmania parasites is critical for evaluating visceral leishmaniasis (VL) treatment response at an early timepoint. We compared the decay of kinetoplast DNA (kDNA) and spliced-leader RNA (SL-RNA) in vitro, in vivo, and in a VL patient cohort. An optimized combination of blood preservation and nucleic acid extraction improved efficiency for both targets. SL-RNA degraded more rapidly during treatment than kDNA, and correlated better with microscopic examination. SL-RNA quantitative polymerase chain reaction emerges as a superior method for dynamic monitoring of viable Leishmania parasites. It enables individualized treatment monitoring for improved prognoses and has potential as an early surrogate endpoint in clinical trials.
- Subjects
BLOOD collection; VISCERAL leishmaniasis; POLYMERASE chain reaction; BIOMARKERS; NUCLEIC acids
- Publication
Journal of Infectious Diseases, 2024, Vol 230, Issue 1, p183
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/jiae219