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- Title
Evaluation of the Treatment Effects of Conditioned Medium from Human Orbital Adipose-Derived Stem Cells in a Corneal Alkali Burn Rabbit Model.
- Authors
Cheng, Yu; Liu, Guangpeng
- Abstract
Purpose: This study aimed to evaluate the effects of a new treatment—conditioned medium from human orbital adipose-derived stem cells (OASC-CM)—on corneal recovery after alkali burns in a rabbit model. Methods: The corneal alkali burn rabbit model was established and treated with OASC-CM, conditioned medium from human abdominal subcutaneous adipose-derived stem cells (ABASC-CM), and fresh control culture medium (con-CM) three times a day for 7 days, respectively. Subsequently, the treatment effects were evaluated and compared through clinical, histological, immunohistochemical, and cytokine evaluations. Results: Clinically, OASC-CM alleviated corneal opacity and edema and promoted recovery of corneal epithelium defect. Histologically and immunohistochemically, OASC-CM inhibited neovascularization, conjunctivalization, and immuno-inflammatory reaction, while promoting corneal regeneration and rearrangement. Increased secretion of interleukin-10 and inhibited protein levels of cluster of differentiation 45, interferon-γ, and tumor necrosis factor-α were observed in the alkali-burned cornea after OASC-CM treatment, which might be the relevant molecular mechanism. Conclusions: OASC-CM showed significant effects on the recovery of rabbit corneal alkali burns and eliminated immunological and ethical limitations, representing a new option for corneal wound treatment.
- Subjects
STEM cells; CORNEA; RABBITS; ALKALIES; CORNEAL opacity; ADIPOSE tissue physiology; WOUND healing
- Publication
Journal of Ocular Pharmacology & Therapeutics, 2024, Vol 40, Issue 4, p222
- ISSN
1080-7683
- Publication type
Article
- DOI
10.1089/jop.2023.0154