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- Title
Adult human pancreatic duct and islet cells exhibit similarities in expression and differences in phosphorylation and complex formation of the homeodomain protein Ipf-1.
- Authors
Heimberg, Harry; Bouwens, Luc; Heremans, Yves; Van De Casteele, Mark; Lefebvre, Veronique; Pipeleers, Daniel; Heimberg, H; Bouwens, L; Heremans, Y; Van De Casteele, M; Lefebvre, V; Pipeleers, D
- Abstract
The homeodomain transcription factor encoded by the pancreatic and duodenal homeobox gene-1 (Ipf-1) is essential for pancreatic ontogenesis. Whether Ipf-1 is also involved in the neogenesis of beta-cells in the adult pancreas is unknown. We examined whether Ipf-1 is expressed in adult human pancreatic ducts, which are thought to generate new beta-cells. In tissue sections, virtually all duct cells were immunopositive for Ipf-1, as were the islet beta-cells but not the acinar cells. After isolation and culture, both duct and islet cell preparations contained the Ipf-1 immunoreactive proteins p42 and p45 (42 and 45 kDa, respectively) in similar proportions, but the expression levels were twofold lower in duct cells. After 4 h of labeling, the endocrine cells exhibited a sevenfold higher phosphorylation of p42 than the duct cells, whereas p45 was phosphorylated only in endocrine cells. Homeobox binding transcription factor complexes with Ipf-1 in duct cells differed from those in endocrine cells in terms of gel mobility, sequence specificity, and affinity. The observed similarities in Ipf-1 expression by adult human pancreatic duct cells and endocrine cells may reflect their common ontogenic origin, whereas the differences in Ipf-1 phosphorylation and complex formation may correlate with their divergent differentiation.
- Subjects
PROTEINS; PHOSPHORYLATION; PANCREATIC duct; ISLANDS of Langerhans; SECRETION; PHYSIOLOGY; PROTEIN metabolism; COMPARATIVE studies; GENE expression; RESEARCH methodology; MEDICAL cooperation; PANCREAS; RESEARCH; EVALUATION research
- Publication
Diabetes, 2000, Vol 49, Issue 4, p571
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diabetes.49.4.571