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- Title
Tripchlorolide ameliorates experimental autoimmune encephalomyelitis by down-regulating ERK1/2-NF-κB and JAK/ STAT signaling pathways.
- Authors
Zhang, Jian; Zeng, Yu‐qi; Zhang, Jing; Pan, Xiao‐dong; Kang, De‐yong; Huang, Tian‐wen; Chen, Xiao‐chun
- Abstract
Tripchlorolide (T4), an extract of the natural herb Tripterygium wilfordii Hook F, has been found to possess anti-inflammatory and immunosuppressive actions. In the current study, these actions were evaluated in experimental autoimmune encephalomyelitis ( EAE), an animal model of multiple sclerosis by scoring the clinical signs, observing the infiltration of inflammatory cells and myelin sheath in the lumbar spinal cord of EAE mice. The results demonstrated that T4 (at a dose of 40 μg/kg) significantly reduced the severity of EAE and slowed down the ongoing EAE. Further analysis showed that T4 suppressed the mRNA and protein levels of the transcription factors T-bet and RoRrt and mRNA levels of IFN-γ and IL-17 in the spinal cords. Furthermore, T4 down-regulated the ERK1/2- NF-κB and JAK/ STAT signaling pathways. At 40 μg/kg, T4 did not induce side effects on hematological parameters. These findings suggest that T4 ameliorates EAE by immunosuppression, providing a new insight into T4 application in multiple sclerosis treatment.
- Subjects
ENCEPHALOMYELITIS; TRIPTERYGIUM wilfordii; AUTOIMMUNE diseases; DOWNREGULATION; EXTRACELLULAR signal-regulated kinases; NF-kappa B; JANUS kinases; THERAPEUTICS
- Publication
Journal of Neurochemistry, 2015, Vol 133, Issue 1, p104
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/jnc.13058