We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Nesfatin-1 activates cardiac vagal neurons of nucleus ambiguus and elicits bradycardia in conscious rats.
- Authors
Brailoiu, G. Cristina; Deliu, Elena; Tica, Andrei A.; Rabinowitz, Joseph E.; Tilley, Douglas G.; Benamar, Khalid; Koch, Walter J.; Brailoiu, Eugen
- Abstract
Nesfatin-1, a peptide whose receptor is yet to be identified, has been involved in the modulation of feeding, stress, and metabolic responses. More recently, increasing evidence supports a modulatory role for nesfatin-1 in autonomic and cardiovascular activity. This study was undertaken to test if the expression of nesfatin-1 in the nucleus ambiguus, a key site for parasympathetic cardiac control, may be correlated with a functional role. As we have previously demonstrated that nesfatin-1 elicits Ca2+ signaling in hypothalamic neurons, we first assessed the effect of this peptide on cytosolic Ca2+ in cardiac pre-ganglionic neurons of nucleus ambiguus. We provide evidence that nesfatin-1 increases cytosolic Ca2+ concentration via a Gi/o-coupled mechanism. The nesfatin-1-induced Ca2+ rise is critically dependent on Ca2+ influx via P/Q-type voltage-activated Ca2+ channels. Repeated administration of nesfatin-1 leads to tachyphylaxis. Furthermore, nesfatin-1 produces a dose-dependent depolarization of cardiac vagal neurons via a Gi/o-coupled mechanism. In vivo studies, using telemetric and tail-cuff monitoring of heart rate and blood pressure, indicate that microinjection of nesfatin-1 into the nucleus ambiguus produces bradycardia not accompanied by a change in blood pressure in conscious rats. Taken together, our results identify for the first time that nesfatin-1 decreases heart rate by activating cardiac vagal neurons of nucleus ambiguus.
- Subjects
BRADYCARDIA; LABORATORY rats; CARDIOVASCULAR disease nursing; PARASYMPATHETIC nervous system; HEART beat; TACHYPHYLAXIS
- Publication
Journal of Neurochemistry, 2013, Vol 126, Issue 6, p739
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/jnc.12355