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- Title
Translocations used to generate chromosome segment duplications in Neurospora can disrupt genes and create novel open reading frames.
- Authors
Singh, Parmit K.; Iyer, Srividhya V.; Sowjanya, T. Naga; Raj, B. Kranthi; Kasbekar, Durgadas P.
- Abstract
In Neurospora crassa, crosses between normal sequence strains and strains bearing some translocations can yield progeny bearing a duplication ( Dp) of the translocated chromosome segment. Here, 30 breakpoint junction sequences of 12 Dp-generating translocations were determined. The breakpoints disrupted 13 genes (including predicted genes), and created 10 novel open reading frames. Insertion of sequences from LG III into LG I as translocation T(UK8-18) disrupts the eat-3 gene, which is the ortholog of the Podospora anserine gene ami1. Since ami1-homozygous Podospora crosses were reported to increase the frequency of repeat-induced point mutation (RIP), we performed crosses homozygous for a deficiency in eat-3 to test for a corresponding increase in RIP frequency. However, our results suggested that, unlike in Podospora, the eat-3 gene might be essential for ascus development in Neurospora. Duplication-heterozygous crosses are generally barren in Neurospora; however, by using molecular probes developed in this study, we could identify Dp segregants from two different translocation-heterozygous crosses, and using these we found that the barren phenotype of at least some duplication-heterozygous crosses was incompletely penetrant.
- Subjects
NEUROSPORA crassa; CHROMOSOME replication; CHROMOSOMAL translocation; PREDICTION models; ANSERINE; GENETIC mutation; PHENOTYPES
- Publication
Journal of Biosciences, 2010, Vol 35, Issue 4, p539
- ISSN
0250-5991
- Publication type
Article
- DOI
10.1007/s12038-010-0062-y