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- Title
Biological Effects of a New Vacuolar-H,<sup>+</sup>-ATPase Inhibitor in Colon Carcinoma Cell Lines.
- Authors
Supino, Rosanna; Scovassi, A. Ivana; Croce, Anna Cleta; Bo, Laura Dal; Favini, Enrica; Corbelli, Alessandro; Farina, Carlo; Misiano, Paola; Zunino, Franco
- Abstract
Vacuolar-H+-ATPase plays a critical role in the cellular balance of protons, thus regulating intracellular pH and contributing to apoptosis resistance, drug resistance, and invasive and metastatic behavior of cells. NiK-12192, a vacuolar-H+-ATPase inhibitor, caused a reduction in the volume and/or acidity of lysosomes, a polarization of αvβ5 integrin distribution, and a number of floating live cells, whereas signs of apoptosis appeared only after 72 h of treatment. In conclusion, NiK-12192, by affecting vacuolar- H+-ATPase activity (and intracellular pH), causes a modification of structures crucial for cell adhesion and induces cell death, likely by a modality involving an anoikis-mediated apoptosis.
- Subjects
CELL lines; ADENOSINE triphosphate; DRUG resistance; MEMBRANE fusion; JUNCTIONAL complexes (Epithelium)
- Publication
Annals of the New York Academy of Sciences, 2009, Vol 1171, p606
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1111/j.1749-6632.2009.04705.x