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- Title
Vitellogenesis in the Red Crab, Charybdis feriatus: Contributions from Small Vitellogenin Transcripts (CfVg) and Farnesoic Acid Stimulation of CfVg Expression.
- Authors
CHAN, SIU‐MING; MAK, ABBY S. C.; CHOI, CHI LUNG; MA, TRACY H. T.; HUI, JEROME H. L.; TIU, SHIRLEY H. K.
- Abstract
During reproductive maturation of the female red crab, Charybdis feriatus, the oocytes rapidly accumulate 110- and 78-kDa major polypeptides. Although the hepatopancreas expresses a high level of vitellogenin (CfVg) mRNA, tissue proteins and secreted proteins of the hepatopancreas consist of only small polypeptides. In addition to the 8.0-kb transcripts, many smaller mRNAs specific to the CfVg gene can be detected. These results suggest that the hepatopancreas also produces smaller CfVg transcripts for small CfVg subunits. Using an RT-PCR cloning approach, a population of the small cDNA clones were isolated. Determining the DNA sequence of these clones revealed that these transcripts were most likely the result of alternative splicing and/or alternative expression of the CfVg gene. In vitro treatment of the hepatopancreas fragments with low levels of farnesoic acid stimulated the expression of CfVg.
- Subjects
CHACEON; PEPTIDE hormones; MESSENGER RNA; GENE expression; NUCLEOTIDE sequence
- Publication
Annals of the New York Academy of Sciences, 2005, Vol 1040, Issue 1, p74
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1327.008