We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Role of Oxidative Stress in Osteoblasts Exposed to Sodium Fluoride.
- Authors
Chun-hong Wang; Zhi-tao Zhao; Wen-bo Zhang; Guang-sheng Li
- Abstract
Abstracts We investigated the relationship between oxidative stress and osteoblasts viability in osteoblasts exposed to various concentrations of fluoride in this study. Primary calvarial osteoblasts from neonatal Kunming mice were cultured and subcultured to the third generation. Osteoblasts were incubated with sodium fluoride (0, 0.5, 1, 2, 4, 8, 12, and 20 mgF−/L) for 24, 48, and 72 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis showed cell viability significantly increased after osteoblasts exposed to low concentrations of fluoride (0.5∼2 mgF−/L) for 24∼72 h. Oxidative stress analysis showed that low concentration of fluoride excited lipid peroxidation in osteoblasts and increased activity of antioxidant enzymes in varying degrees. We demonstrated that changes of osteoblasts viability of the low-dose fluoride groups are different from those of high-dose fluoride groups; however, both low and high doses of fluoride caused active state of oxidative stress in osteoblasts, which suggesting that oxidative stress may be excited by the active osteoblasts viability induced by a low dose of fluoride.
- Subjects
PEROXIDATION; OXIDATIVE stress; SODIUM fluoride; FLUORIDES; OXIDATION; THERAPEUTICS
- Publication
Biological Trace Element Research, 2008, Vol 123, Issue 1-3, p109
- ISSN
0163-4984
- Publication type
Article