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- Title
Comparative metagenomic discovery of the dynamic cellulose-degrading process from a synergistic cellulolytic microbiota.
- Authors
Yang, Ming; Zhao, Jingjing; Yuan, Yue; Chen, Xiaoyi; Yang, Fan; Li, Xianzhen
- Abstract
To reveal the dynamic process of cellulose biodegradation and explore more potential cellulases, a microbiota (FPDM) with cellulose-degrading ability was cultivated, and different stages of filter paper degradation were compared. Ion chromatography and comparative metagenomic sequencing revealed that the diversity of FPDM enhanced as the hydrolysate length diversity increased. Sporocytophaga and Cohnella dynamically dominated the synergistic degradation of cellulose in early-intermediate and intermediate-final periods, respectively. Moreover, 363 declining shifting and 231 progressive shifting unannotated genes were speculated to participate in the catabolism of cellulose to cellodextrin/cello-oligosaccharide and to cellobiose, respectively. Based on the dynamic changes in hydrolysates, community structure and gene abundance, a dynamic cellulose-degrading pathway of FPDM was predicted. Our work should provide a new perspective for subsequent identification of key cellulolytic strains and enzymes and clarification of the mechanism of cellulose biodegradation.
- Publication
Cellulose, 2021, Vol 28, Issue 4, p2105
- ISSN
0969-0239
- Publication type
Article
- DOI
10.1007/s10570-020-03671-z