We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Thermal inactivation of volume-sensitive K,Cl cotransport and plasma membrane relief changes in human erythrocytes.
- Authors
Parshina, E.; Yusipovich, A.; Platonova, A.; Grygorczyk, R.; Maksimov, G.; Orlov, S.
- Abstract
Previously, we reported that in mammalian erythrocytes irreversible annealing of spectrin heterodimers at 49-50 °C abolished cell volume-dependent regulation of ion carriers, thus suggesting an implication of a two-dimensional (2D) membrane carcass in volume sensing and/or signal transduction. To further examine this hypothesis, we employed atomic force microscopy. This method revealed folded membrane relief of fixed human erythrocytes with an average wave height of 3-5 nm covered by globular structures with a diameter of 40-50 nm and an average height of 1-2 nm. Erythrocyte swelling caused by reduction of medium osmolality decreased the height of membrane surface waves by 40 % and increased K,Cl cotransport by approximately sixfold. Both volume-sensitive changes of membrane relief and activity of K,Cl cotransporter were abolished by a 10-min preincubation at 50 °C. Our results strongly suggest that volume-dependent alterations of the human erythrocyte membrane relief are caused by reorganization of the 2D spectrin-actin network contributing to regulation of the activity of volume-sensitive ion transporters.
- Subjects
CELL membranes; ERYTHROCYTES; DIMERS; CELL size; ATOMIC force microscopy
- Publication
Pflügers Archiv: European Journal of Physiology, 2013, Vol 465, Issue 7, p977
- ISSN
0031-6768
- Publication type
Article
- DOI
10.1007/s00424-013-1221-4