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- Title
Development of user-friendly markers for disease resistance to black root rot of tobacco through genotyping by sequencing.
- Authors
Qin, Qiulin; Li, Yuting; Ding, Na; Li, Dandan; Martinez, Natalia; Miller, Robert; Zaitlin, David; Yang, Shengming
- Abstract
Black root rot (BRR), a disease caused by the hemibiotrophic fungus Thielaviopsis basicola, seriously compromises yield and leaf quality in tobacco (Nicotiana tabacum). Full resistance to black root rot, conferred by the resistance to BRR 1 (RBRR1) locus from Nicotiana debneyi Domin, was transferred to a burley tobacco cultivar through interspecific hybridization. Some undesirable traits potentially caused by linkage drag restrict wider application of RBRR1 in flue-cured tobacco. Therefore, user-friendly molecular markers are needed to assist selection for resistance to black root rot and to break the unfavorable linkage. Genotyping by sequencing (GBS) is a rapid and robust approach for reduced representation sequencing of multiplexed genomic DNA samples that combines genome-wide molecular marker discovery with genotyping. In the present study, we used GBS to identify single-nucleotide polymorphisms (SNPs) linked to the RBRR1 locus, and PCR-based assays for detection of these SNPs were also developed. Sequence analysis of the SNP markers suggested that RBRR1 is located on chromosome 17, providing a basis for map-based cloning of this valuable gene. Co-dominant CAPS markers that co-segregate with the disease-resistant phenotype offer user-friendly tools for tobacco breeding and variety improvement. Furthermore, tested with diverse N. tabacum germplasm, SS192650 displayed 100% selection accuracy for resistance to BRR, suggesting that this marker can be used in diverse tobacco populations.
- Subjects
GENETIC markers in plants; TOBACCO disease &; pest resistance; THIELAVIOPSIS basicola; TOBACCO yields; PLANT hybridization
- Publication
Molecular Breeding, 2018, Vol 38, Issue 6, p1
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-018-0834-x