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- Title
Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells.
- Authors
Kowalik, Klaudia; Paduch, Roman; Strawa, Jakub W.; Wiater, Adrian; Wlizło, Kamila; Waśko, Adam; Wertel, Iwona; Pawłowska, Anna; Tomczykowa, Monika; Tomczyk, Michał; Capasso, Raffaele
- Abstract
The aim of this study was to determine the anti-tumor activity of extracts isolated from Potentilla alba L. on human colon cancer cells of the HT-29 line and on non-cancer colon epithelial cells of the CCD 841 CoTr line. The research methods we used to determine the cytotoxic and proliferative properties were 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, the ability to produce nitric oxide, the Griess method, and the biochemical properties like 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods indicating reduction activity of tested samples. In order to obtain a phytochemical profile of the different extracts an analytical method based on liquid chromatography-photodiode array detection-electrospray ionization ion-trap time-of-flight mass spectrometry (LC-PDA-ESI-MS/TOF) was applied. Finally, the effects of the extracts on the morphology and cell counts were assessed by May–Grünwald–Giemsa staining. After a comprehensive analysis of all the experiments, the extracts were found to demonstrate cytotoxic properties, they stimulated the division of non-cancer cells, and they were able to scavenge free radicals. In the NR method, the cell viability dropped to approximately 80% compared to the control. In the MTT assay, tumor cell proliferation decreased to 9.5% compared to the control. Therefore, we concluded that this plant has medical potential.
- Subjects
COLON (Anatomy); EPITHELIAL cells; TIME-of-flight mass spectrometry; CELL morphology; EXTRACTS; CELL-mediated cytotoxicity; CELL division; BOTANICAL chemistry
- Publication
Molecules, 2020, Vol 25, Issue 13, p3080
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules25133080