We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Dataset: Impact of β-Galactosylceramidase Overexpression on the Protein Profile of Braf(V600E) Mutated Melanoma Cells.
- Authors
Capoferri, Davide; Chiodelli, Paola; Calza, Stefano; Manfredi, Marcello; Presta, Marco
- Abstract
β-Galactosylceramidase (GALC) is a lysosomal enzyme involved in sphingolipid metabolism by removing β-galactosyl moieties from β-galactosyl ceramide and β-galactosyl sphingosine. Previous observations have shown that GALC exerts a pro-oncogenic activity in human melanoma. Here, the impact of GALC overexpression on the proteomic landscape of BRAF-mutated A2058 and A375 human melanoma cell lines was investigated by liquid chromatography–tandem mass spectrometry analysis of the cell extracts. The results indicate that GALC overexpression causes the upregulation/downregulation of 172/99 proteins in GALC-transduced cells when compared to control cells. Gene ontology categorization of up/down-regulated proteins indicates that GALC may modulate the protein landscape in BRAF-mutated melanoma cells by affecting various biological processes, including RNA metabolism, cell organelle fate, and intracellular redox status. Overall, these data provide further insights into the pro-oncogenic functions of the sphingolipid metabolizing enzyme GALC in human melanoma. Dataset: The data set has been submitted as a supplement to this paper. Dataset License: license under which the dataset is made available (CC0, CC-BY, CC-BY-SA, CC-BY-NC, etc.)
- Subjects
PROTEIN overexpression; RNA metabolism; LIQUID chromatography-mass spectrometry; BRAF genes; MELANOMA
- Publication
Data (2306-5729), 2023, Vol 8, Issue 12, p177
- ISSN
2306-5729
- Publication type
Article
- DOI
10.3390/data8120177