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- Title
NOVEL BIFIDOCIN LHM3 : MILK PRESERVATION AND ANTIBACTERIAL ACTIVITY AGAINST ENTEROBACTER SAKAZAKII AND BACILLUS SUBTILIS.
- Authors
Mahdi Al- Moosawi, Likaa Hamied; Mahmood, Nibras Nazar; Hussein Al-Kakei, Sana’a Noori; Musafer, Hadeel
- Abstract
Seven isolates belonged to Bifidobacterium longum isolated from mother breast milk and their infant feces. These isolates were identified by biochemical tests and confirmed by Api 50. After that, they screened for higher bacteriocin production. Five isolates of Enterobacter sakazakii isolated from newborn milk powder and 2 isolates of Bacillus subtilis isolated from newborn milk powder and infant hard food. A three-step method of purification was done for new bacteriocin purification, from B. longum called bifidocin LHM3. The purification revealed to a purification fold of 3.2049 with a specific activity of 1500000 AU/mg and a yield of 0.1%. Bacteriocin was obtained by desorption - adsorption on / from silicic acid. Bifidobacterium bacteriocin is protein in nature, is adiabatic and active in a wide range of pH values, molecular weight of the bacteriocin was 14. Bacteriocin was obtained by desorption - adsorption on / from silicic acid 5KDa Bacteriocin. It can be classified as the second type, it was decreased to 480 Au/ml when treated with the chloroform. Limited studies have shown that bacteriocin from several Bifidobacterium longum isolates can be also used effectively in controlling food systems. This study showed the potential of B. longum bacteriocin in biological preservation of milk. We anticipated that milk preservation by bacteriocin positive results; potentially benefit both the producer and consumer. B. longum crude and purified bacteriocin had antibacterial activity against both E. sakazakii and B. subtilis with highest activity at 500μg/ml and the purified bacteriocin recorded higher antibacterial activity.
- Subjects
BIFIDOBACTERIUM longum; ANTIBACTERIAL agents; ENTEROBACTER sakazakii; BACILLUS subtilis; BACTERIOCINS
- Publication
Biochemical & Cellular Archives, 2021, Vol 21, Issue 1, p1221
- ISSN
0972-5075
- Publication type
Article