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- Title
L-酪氨酸连续发酵工艺研究.
- Authors
王锐麒; 刘韪玮; 赵春光; 徐庆阳
- Abstract
In the current industrial process of producing L-tyrosine by microbial fermentation method, the basic culture medium is generally added into the fermentation tank in the early stage, and then various nutrients are fed in the middle and later stages. Although in the entire fermentation process, the operations of taking and releasing materials are reduced, ensuring that materials are not wasted during the fermentation, with the continuous increase of fed-in materials, the fermentation liquid system continues to expand. The fermentation parameters need to be continuously regulated and controlled artificially in the later stage of fermentation. However, it is difficult to avoid the fluctuation of the regulation parameters in the artificial regulation and control, and eventually affecting the fermentation results. Therefore, efficient continuous fermentation is adopted in this experiment, which can greatly improve the cell vitality and effectively prolong the peak period of acid production. In order to optimize the high-density continuous fermentation production process of L-tyrosine, the fermentation process of L-tyrosine is investigated through the fermentation culture of E. coli TYR-05, and the biomass, acid production amount, acid production efficiency and sugar-acid conversion rate are analyzed. Key conditions such as inoculation amount, sugar rate, sugar consumption amount and base sugar concentration are determined during the high-density continuous fermentation process of L-tyrosine, in order to achieve the optimization of the control conditions for the high-density continuous fermentation process. The experimental results show that in a 5 L fermentation tank, 30% seed inoculation amount is selected. At the beginning of fermentation, the substrate choline chloride concentration is 1 g/L, and 0. 2 g/L choline chloride is fed into the tank every 4 h. After 12 h of fermentation, the base sugar is depleted, and glucose is supplied into the tank at a sugar supplement rate of 12 g/(L·h). At this time, the liquid is released at a rate of 0. 13 L/h to make the liquid volume constant at about 20%. After 25 h of fermentation, compound nutrient liquid is fed in. After 35 h of fermentation, the highest cell OD600, reaches 65, the acid production amount is 55. 8 g/L, and the sugar-acid conversion rate is 25. 4%, which has provided important references for the industrial production of L-tyrosine continuous fermentation.
- Subjects
FERMENTATION; MICROBIOLOGICAL synthesis; ESCHERICHIA coli; TYROSINE; AMINO acids
- Publication
China Condiment, 2024, Vol 49, Issue 1, p107
- ISSN
1000-9973
- Publication type
Article
- DOI
10.3969/j.issn.1000-9973.2024.01.018