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- Title
Ginsenoside Rb1 protects porcine oocytes against methylglyoxal damage thus it improves the quality of parthenogenetic activation and in vitro fertilization embryos.
- Authors
Luo, Zhao‐Bo; Xuan, Mei‐Fu; Han, Sheng‐Zhong; Li, Zhou‐Yan; Khan, Nasar; Quan, Biao‐Hu; Yin, Xi‐Jun; Kang, Jin‐Dan
- Abstract
Panax ginseng, a functional food, has been widely used as an edible nourishment and medicinal supplement. Ginsenoside Rb1 is a major bioactive ingredient of ginseng, which shows very specific anti‐apoptosis and anti‐oxidant activities. Methylglyoxal (MGO) is one of intermediate products of glucose metabolism, which is absorbed easily from high sugar foods or carbonated beverages. It may involve in a variety of detrimental processes in vivo. However, it has not been fully explored the effects of ginsenoside Rb1 on MGO‐induced oocytes damage. This study found that MGO‐induced DNA damage and mitochondrial dysfunction result in the failure of porcine oocytes maturation and low in vitro development capacity of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos. Conversely, Rb1 supplementation recovered the rate of maturation, and improved in vitro development capacity of PA and IVF embryos. Rb1 also provided porcine oocytes a lower level of reactive oxygen species production, higher level of ATP content and mitochondrial membrane potential, and stimulated pluripotency gene expression in blastocysts. The findings of this study reveal ginsenoside Rb1 protects porcine oocyte from the cytotoxicity effects of methylglyoxal and provides novel perspectives for the protection of reproduction system by functional food of ginseng.
- Subjects
OVUM; FERTILIZATION in vitro; HUMAN in vitro fertilization; PYRUVALDEHYDE; GINSENG; EMBRYOS; REACTIVE oxygen species
- Publication
Environmental Toxicology, 2021, Vol 36, Issue 4, p586
- ISSN
1520-4081
- Publication type
Article
- DOI
10.1002/tox.23063