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- Title
Effect of 5-S-GAD on UV-B-induced cataracts in rats.
- Authors
Takahito Kimura; Shunji Natori
- Abstract
Abstract Purpose 5-S-Glutathionyl-N-β-alanyl-3,4-dihydroxyphenylalanine (5-S-GAD) is a novel antibacterial substance purified from Sarcophaga peregrina (flesh fly) that has both a radical scavenging activity and antioxidative activity. This is a report of an investigation of the effect of 5-S-GAD (eyedrops) on UVB-induced cataracts in rats. Methods Brown Norway male rats (n = 32; 7 weeks old) were treated with either 5-S-GAD 0.1%, 5-SGAD 1%, astaxanthin (AST) 0.1% suspension eyedrops or the vehicle alone (the solution without 5-S-GAD) three times a day (three doses at 5-min intervals each time). The treatment was scheduled 2 days before UV-B exposure and 2 days after UV-B exposure. Exposure to 100–200 mJ/cm2 UV-B was performed once a week between drug treatments for 9 consecutive weeks, with a total dose of 1200 mJ/cm2 UV-B. Ocular penetration of 5-S-GAD was analyzed using high-pressure liquid chromatography (HPLC). Cataract formation was documented by an anterior eye segment analysis system once a week under mydriasis. The light-scattering intensity (LSI) of the anterior superficial cortex region was measured. Results In the eighth to ninth week after the start of UV-B exposure, the LSI of anterior subcapsular lenses of 5-S-GAD-treated groups, as detected by HPLC, was significantly lower (P Conclusion 5-S-GAD eyedrop application may delay the progression of UV-B-induced cataract in rats.
- Subjects
ANTIBACTERIAL agents; DOPA; HIGH performance liquid chromatography; ANTERIOR eye segment; LIGHT scattering; EYE diseases; LABORATORY rats
- Publication
Japanese Journal of Ophthalmology, 2009, Vol 53, Issue 5, p531
- ISSN
0021-5155
- Publication type
Article