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- Title
Techniques for extraction of depth-resolved in vivo human retinal intrinsic optical signals with optical coherence tomography.
- Authors
Tumlinson, Alexandre R.; Hermann, Boris; Hofer, Bernd; Považay, Boris; Margrain, Tom H.; Binns, Alison M.; Drexler, Wolfgang
- Abstract
Purpose: To demonstrate acquisition and analysis methods for depth-resolved observation of slow retinal physiology induced changes in infrared backscatter in vivo. Methods: A dark-adapted human was briefly subjected to a localized photobleach. For 20 min before and 30 min after the stimulus, volumetric optical coherence tomograms were collected partially overlapping the bleached region. Tomograms were segmented into retinal layers by a newly described algorithm exploiting information in adjacent B-scans. En face fundus images extracted from major intraretinal layers were laterally registered manually. Time series summarizing the observed backscatter in selected layers for the bleached and unbleached areas are shown with a variety of corrections and normalizations applied: tomograms were corrected for inherent sensitivity roll-off, and the ratio between other layers and an assumed unchanging layer (retinal pigment epithelium), as well as the ratio of the stimulated area to the unstimulated area, were calculated. Results: Adjacent B-scan information allows a simpler segmentation algorithm to be used. Sensitivity roll-off correction reduces signal variability due to eye motion. After normalizations, the signal correlated with the stimulus appears strongest at the photoreceptor inner-outer segment junction. Conclusions: Demonstrated methods manage data complexity and reduce uncorrelated signal variability. This single trial warrants further investigation of intrinsic optical signals to observe slow physiologic responses.
- Subjects
RETINA; OPTICAL coherence tomography; FUNDUS oculi; PHOTORECEPTORS; TOMOGRAPHY
- Publication
Japanese Journal of Ophthalmology, 2009, Vol 53, Issue 4, p315
- ISSN
0021-5155
- Publication type
Article
- DOI
10.1007/s10384-009-0684-5