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- Title
Expression and Distribution of Junctional Adhesion Molecule-1 in the Human Cornea.
- Authors
Nobuyuki Ebihara; Keiko Fujiki; Akira Murakami
- Abstract
Abstract Purpose To investigate the expression and distribution of junctional adhesion molecule 1 (JAM-1) in human corneal tissue and cells. Methods Reverse transcriptase-polymerase chain reaction was used to detect the expression of JAM-1, ZO-1, and occludin mRNAs in corneal cells, while the presence of JAM-1 protein was analyzed by flow cytometry (FACS). Double immunofluorescence staining was used to determine the tissue distribution of JAM-1 and occludin in human corneas. Results Strong expression of JAM-1, ZO-1, and occludin mRNAs was observed in primary cultured corneal epithelial and endothelial cells, but not in primary cultured keratocytes. The expression of JAM-1 protein in cultured epithelial and endothelial cells was confirmed by FACS. When keratocytes were cultured in medium with 10% fetal calf serum for several passages, differentiation into corneal myofibroblasts occurred. The expression of JAM-1 was detected in these corneal myofibroblasts at both RNA and protein levels. JAM-1 immunoreactivity was seen at cell borders throughout the entire epithelium, but not in keratocytes from normal corneal tissue. On the other hand, JAM-1 immunoreactivity was detected in the cytoplasm of corneal endothelial cells. Jpn J Ophthalmol 2007;51:405–411 © Japanese Ophthalmological Society 2007 Conclusions JAM-1 is expressed by human corneal epithelial and endothelial cells, but not by keratocytes, although its expression is induced in corneal myofibroblasts.
- Subjects
CORNEA; CELL adhesion molecules; TISSUES; CELLS; POLYMERASE chain reaction; MESSENGER RNA
- Publication
Japanese Journal of Ophthalmology, 2007, Vol 51, Issue 6, p405
- ISSN
0021-5155
- Publication type
Article