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- Title
Elevation of Intracellular Ca<sup>2+</sup> Concentration Induced by Hypoxia in Retinal Ganglion Cells.
- Authors
Akimichi Kaneko
- Abstract
Abstract Purpose To analyze the mechanism of hypoxia-induced changes of the intracellular Ca2+ concentration ([Ca2+]i) in retinal ganglion cells (RGCs). Methods Fluo-3 was applied to the cut edge of the optic nerve of 6-week-old rats. The retina was sliced, and the Ca images were captured. A hypoxic condition was created by superfusing the retinal slice with an oxygen/glucose-deprived solution. Results The retrograde staining method filled the RGCs selectively. Fifteen minutes of hypoxic conditions induced an increase in [Ca2+]i in the RGCs (Δ0.13 0.03, n = 23). Application of 60 M dl-2-amino-5-phosphonovaleric acid partially blocked the hypoxia-induced [Ca2+]i increase in dendrites (Δ0.03 0.02, n = 4, P n = 9). The RGC dendrites showed a further increase in [Ca2+]i after being switched back to an oxygenated solution (Δ0.14 0.04, n = 4). Neither 6-cyano-7-nitroquinoxaline-2,3-dione disodium, dl-threo-β-benzyloxyaspartate, nifedipine, nor bepridil inhibited the hypoxia-induced [Ca2+]i increase. A Ca2+-free superfusion prevented the hypoxia-induced [Ca2+]i increase in the somata (Δ0.07 0.02, n = 5, P n = 4). Conclusions The mechanism of the hypoxia-induced increase in [Ca2+]i differs between somata and dendrites. The N-methyl-d-aspartate channel of dendrites seems to be the main route of Ca2+ influx. Jpn J Ophthalmol 2007;51:175–180 Japanese Ophthalmological Society 2007
- Subjects
HYPOXEMIA; RETINAL ganglion cells; OXYGEN; LABORATORY rats; DENDRITES
- Publication
Japanese Journal of Ophthalmology, 2007, Vol 51, Issue 3, p175
- ISSN
0021-5155
- Publication type
Article