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- Title
芦丁通过激活 NRF2/HO-1 通路抑制 Ang II 诱导的 血管平滑肌细胞表型转化和炎症反应.
- Authors
谢孝平; 沈小艳; 李博文; 刘天宇; 王志维
- Abstract
Objective: To investigate the effects and mechanisms of rutin on angiotensin Ⅱ (Ang Ⅱ) -induced phenotype transition and inflammatory response in mouse aortic vascular smooth muscle cells. Methods: Mouse aortic vascular smooth muscle cells in logarithmic growth phase were divided into four groups: Control group (Control), Rutin group (100 μM), Ang Ⅱ group (1 μM), and Rutin+Ang Ⅱ group (100 μM, 1 μM) . Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay to evaluate the effect of rutin on mouse aortic vascular smooth muscle cell viability. Protein immunoblotting was performed to examine the expression and phosphorylation levels of α-smooth muscle actin (α-SMA), Smooth muscle protein 22-alpha (SM22α), Osteopontin (OPN), Matrix metalloproteinase 2 (MMP2), Matrix metalloproteinase 9 (MMP9), Interleukin 6 (IL6), Tumor necrosis factor-alpha (TNF-α), Nuclear factor erythroid 2-related factor 2 (NRf2), Heme oxygenase-1 (HO-1), and Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) .Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of TNF-α and IL6 in the cell supernatant. Fluorescence microscopy and flow cytometry were employed to detect the levels of reactive oxygen species (ROS) in mouse aortic vascular smooth muscle cells. Results: Compared to the control group, the Ang Ⅱ group exhibited decreased expression levels of contractile markers α-SMA and SM22α, increased expression level of synthetic marker OPN, elevated expression levels of inflammatory factors MMP2, MMP9, IL6, and TNF-α, decreased expression levels of NRf2 and HO-1, and increased phosphorylation levels of NRf2 and NF-κB. Additionally, compared to the Ang Ⅱ group, the Rutin+Ang Ⅱ group showed increased expression levels of contractile markers α-SMA and SM22α, decreased expression level of synthetic marker OPN, reduced expression levels of inflammatory factors MMP2, MMP9, IL6, and TNF-α, increased expression levels of NRf2 and HO-1, and decreased phosphorylation levels of NRf2 and NF-κB. Conclusion: Rutin can inhibit Ang Ⅱ-induced phenotype transition and inflammatory response in mouse aortic vascular smooth muscle cells, possibly through activation of the NRF2/HO-1 pathway and inhibition of ROS production.
- Subjects
VASCULAR smooth muscle; MUSCLE cells; OXIDATIVE stress; INFLAMMATION; RUTIN
- Publication
Progress in Modern Biomedicine, 2023, Vol 23, Issue 22, p4201
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2023.22.001