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- Title
Loureirin B inhibits fibroblast proliferation and extracellular matrix deposition in hypertrophic scar via TGF- β/Smad pathway.
- Authors
Bai, Xiaozhi; He, Ting; Liu, Jiaqi; Wang, Yunchuan; Fan, Lei; Tao, Ke; Shi, Jihong; Tang, Chaowu; Su, Linlin; Hu, Dahai
- Abstract
The ethanolic extract of Resina Draconis (RDEE) has been reported beneficial to normal wound healing yielding more regularly arranged collagen fibres. Loureirin B, a major component in RDEE, has been supposed to be effective on the prevention and treatment of pathological scars. To investigate the therapeutic effects of loureirin B on hypertrophic scar (HS), fibroblasts from human HS and normal skin (NS) were isolated. Results showed that loureirin B dose-dependently downregulated both m RNA and protein levels of type I collagen (ColI), type III collagen (ColIII) and α -smooth muscle actin ( α -SMA) in HS fibroblasts. Loureirin B also suppressed fibroblast proliferative activity and redistributed cell cycle, but did not affect cell apoptosis. In vivo rabbit ear scar model, loureirin B significantly improved the arrangement and deposition of collagen fibres, decreased protein levels of ColI, ColIII and α -SMA and suppressed myofibroblast differentiation and scar proliferative activity. In NS fibroblasts, loureirin B effectively inhibited TGF- β1-induced upregulation of ColI, ColIII and α-SMA levels, myofibroblast differentiation and the activation of Smad2 and Smad3. Loureirin B also affected m RNA levels of major MMPs and TIMPs in TGF- β1-stimulated fibroblasts. Taken together, this study demonstrates that loureirin B could downregulate the expression of fibrosis-related molecules by regulating MMPs and TIMPs levels, inhibit scar fibroblast proliferation and suppress TGF- β1-induced fibrosis, during which TGF- β1/Smad2/3 pathway is likely involved. These findings suggest that loureirin B is a potential therapeutic compound for HS treatment.
- Subjects
FIBROBLASTS; CELL proliferation; EXTRACELLULAR matrix; HYPERTROPHIC scars; TRANSFORMING growth factors; WOUND healing; COLLAGEN; THERAPEUTICS
- Publication
Experimental Dermatology, 2015, Vol 24, Issue 5, p355
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/exd.12665