We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Vasculogenic properties of adventitial Sca-1<sup>+</sup>CD45<sup>+</sup> progenitor cells in mice: a potential source of vasa vasorum in atherosclerosis.
- Authors
Toledo-Flores, Deborah; Williamson, Anna; Schwarz, Nisha; Fernando, Sanuja; Dimasi, Catherine; Witt, Tyra A.; Nguyen, Thao M.; Puranik, Amrutesh S.; Chue, Colin D.; Delacroix, Sinny; Spoon, Daniel B.; Bonder, Claudine S.; Bursill, Christina A.; Di Bartolo, Belinda A.; Nicholls, Stephen J.; Simari, Robert D.; Psaltis, Peter J.
- Abstract
The cellular origins of vasa vasorum are ill-defined and may involve circulating or local progenitor cells. We previously discovered that murine aortic adventitia contains Sca-1+CD45+ progenitors that produce macrophages. Here we investigated whether they are also vasculogenic. In aortas of C57BL/6 mice, Sca-1+CD45+ cells were localised to adventitia and lacked surface expression of endothelial markers (<1% for CD31, CD144, TIE-2). In contrast, they did show expression of CD31, CD144, TIE-2 and VEGFR2 in atherosclerotic ApoE−/− aortas. Although Sca-1+CD45+ cells from C57BL/6 aorta did not express CD31, they formed CD31+ colonies in endothelial differentiation media and produced interconnecting vascular-like cords in Matrigel that contained both endothelial cells and a small population of macrophages, which were located at branch points. Transfer of aortic Sca-1+CD45+ cells generated endothelial cells and neovessels de novo in a hindlimb model of ischaemia and resulted in a 50% increase in perfusion compared to cell-free control. Similarly, their injection into the carotid adventitia of ApoE−/− mice produced donor-derived adventitial and peri-adventitial microvessels after atherogenic diet, suggestive of newly formed vasa vasorum. These findings show that beyond its content of macrophage progenitors, adventitial Sca-1+CD45+ cells are also vasculogenic and may be a source of vasa vasorum during atherogenesis.
- Publication
Scientific Reports, 2019, Vol 9, Issue 1, pN.PAG
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-019-43765-8