We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A novel lipid droplets‐targeting fluorescent probe based on dicyanisophorone and carbazole for Cu<sup>2+</sup> and its application.
- Authors
Cui, Yuanyuan; Meng, Yaoyang; Hu, Yun; Chen, Yuqi; Yang, Yixian; Fang, Min; Li, Cun; Zhu, Weiju
- Abstract
A novel fluorescent probe, LCH, based on dicyanisophorone and carbazole, was prepared for the visual detection of Cu2+. The probe LCH could recognize Cu2+ by fluorescence quenching in EtOH/H2O (1/4, v/v) solution, which could be easily identified under the 365 nm UV lamp, and the detection limit was as low as 0.785 μM. The recognition mechanism of probe LCH with Cu2+ was determined by combining 1H NMR titration, MS, and theoretical calculations. Practical application experiments showed that probe LCH could be used to detect Cu2+ in the test strip experiments. Cell imaging experiments showed that the probe LCH owned good cell permeability and could be applied to the imaging of Cu2+ in HepG2 cells. In addition, fluorescence colocalization experiments showed that LCH could target lipid droplets. These results indicate that the probe LCH will have a good application prospect in environmental detection and clinical medicine.
- Subjects
FLUORESCENT probes; FLUORESCENCE quenching; CELL imaging; ULTRAVIOLET lamps; CELL permeability; CARBAZOLE
- Publication
Journal of the Chinese Chemical Society, 2023, Vol 70, Issue 1, p68
- ISSN
0009-4536
- Publication type
Article
- DOI
10.1002/jccs.202200401