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- Title
Gelsolin: a new biomarker of disease activity in SLE patients associated with HDL-c.
- Authors
Parra, Sandra; Heras, Mercedes; Herrero, Pol; Amigó, Nuria; Garcés, Esperanza; Girona, Josefa; Correig, Xavier; Canela, Nuria; Castro, Antoni
- Abstract
Objectives To identify potential biomarkers of disease activity analysing the proteome of high-density lipoprotein (HDL) particles from SLE patients in clinical remission and when they develop a flare compared with a healthy control group. Methods Quantitative proteomic analyses of purified HDL were performed using Tandem Mass Tag isobaric tag-labelling and nanoLC-Orbitrap (nLC-MS/MS) from nine SLE patients in clinical remission when they developed a flare and from nine healthy controls (9–9-9). We verified the identified proteins by Western blot and ELISA in a cohort of 104 SLE women patients, 46 healthy women and 14 SLE patients when a flare developed. Results We found 17 proteins with a significant fold-change (>1.1) compared with the control group. In lupus patients experiencing a flare compared with those in remission, we identified four proteins with a significant fold-change (C4, Indian Hedgehog protein, S100A8 and gelsolin). Plasma gelsolin (pGSN) levels were decreased in the 104 SLE patients (176.02(74.9) mcg/l) compared with the control group (217.13(86.7) mcg/l); P =0.005 and when they developed a clinical flare (104.84(41.7) mcg/l); P =0.002). pGSN levels were associated with HDL cholesterol levels (r = 0.316, P <0.001). Antimalarial treated patients showed significant higher levels of pGSN (214.56(88.94) mcg/l regarding 170.35(66.36) mcg/l); P = 0.017. Conclusion Decreased pGSN are associated with clinical disease activity in SLE patients. Antimalarial treatment and HDL cholesterol are associated with higher levels of pGSN.
- Subjects
ANTIMALARIALS; BIOMARKERS; ENZYME-linked immunosorbent assay; HIGH density lipoproteins; SYSTEMIC lupus erythematosus; WESTERN immunoblotting; PROTEOMICS; SYMPTOMS; DISEASE remission
- Publication
Rheumatology, 2020, Vol 59, Issue 3, p650
- ISSN
1462-0324
- Publication type
Article
- DOI
10.1093/rheumatology/kez293