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- Title
Rapid detection of kanamycin using cooperative recognition split aptamer and graphene oxide nanosheets.
- Authors
Ye, Hua; Wan, Tianxiang; Li, Xinfu; Li, Chao; He, Kuo; Guo, Yuanxin
- Abstract
Split aptamers are fragments of the parent aptamer which still possess binding ability to its target. They have shown great potential in the development of aptasensors. In this study, a fluorescence aptasensor was constructed employing the split aptamer as a cooperative recognition molecular probe and Graphene Oxide nanosheets (GO) as a quencher for rapid detection of kanamycin in chilled pork. Under optimal conditions,the proposed biosensor showed a good linear relationship in the range of 0.5–50 nM with the detection limit of 0.36 nM. The recoveries of the actual samples ranged from 97.6 to 103.5%. Furthermore, the test can be completed in 25 min. This study provides good reference for developing simple, economical, and rapid assay for other small molecular targets detection.
- Subjects
APTAMERS; MOLECULAR recognition; KANAMYCIN; NANOSTRUCTURED materials; MOLECULAR probes
- Publication
Journal of Food Measurement & Characterization, 2023, Vol 17, Issue 3, p2144
- ISSN
2193-4126
- Publication type
Article
- DOI
10.1007/s11694-022-01781-9