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- Title
A Novel Ratiometric Two-Photon Fluorescent Probe for the Detection of Biothiols in Solution and Imaging of Living Cells.
- Authors
Wu, Qing ‐ Qing; Xiao, Zu ‐ Feng; Du, Xiao ‐ Jiao; Song, Qin ‐ Hua
- Abstract
What a great response: A novel fluorescent probe for biothiols has been constructed based on the Michael addition. The adducts of the probe with thiols possess an intramolecular charge transfer character in their excited states, and detection of biothiols reveals a ratiometric spectral response. Furthermore, biothiols were detected through cell imaging under both one ‐ photon and two ‐ photon excitations.
- Subjects
SPECTRUM analysis; THIOLS; BIOMOLECULES; FLUORESCENT probes; CYSTEINE; MICHAEL reaction; PHOTONS
- Publication
Chemistry - An Asian Journal, 2013, Vol 8, Issue 11, p2564
- ISSN
1861-4728
- Publication type
Article
- DOI
10.1002/asia.201300491