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- Title
Aroma volatiles obtained at harvest by HS-SPME/ GC-MS and INDEX/ MS-E-nose fingerprint discriminate climacteric behaviour in melon fruit.
- Authors
Chaparro‐Torres, Libia A; Bueso, María C; Fernández‐Trujillo, Juan P
- Abstract
BACKGROUND Melon aroma volatiles were extracted at harvest from juice of a climacteric near-isogenic line ( NIL) SC3-5-1 with two quantitative trait loci ( QTLs) introgressed which produced climacteric behaviour and its non-climacteric parental ( PS) using two methodologies of analysis: static headspace solid phase micro-extraction ( HS-SPME) by gas chromatography-mass spectrometry ( GC-MS) and inside needle dynamic extraction ( INDEX) by MS-based electronic nose ( MS-E-nose). RESULTS Of the 137 volatiles compounds identified, most were found at significantly higher concentrations in SC3-5-1 than in PS in both seasons. These volatiles were mostly esters, alcohols, sulfur-derived esters and even some aldehydes and others. The number of variables with high correlation values was reduced by using correlation network analysis. Partial least squares-discriminant analysis ( PLS-DA) achieved the correct classification of PS and SC3-5-1. The ions m/ z 74, 91, 104, 105, 106 and 108, mainly volatile derivatives precursor phenylalanine, were the most discriminant in SC3-5-1 and PS. As many as 104 QTLs were mapped in season 1 and at least 78 QTLs in each season with an effect above the PS mean. CONCLUSION GC-MS gave better discrimination than E-nose. Most of the QTLs that mapped in both seasons enhanced aroma volatiles associated with climacteric behaviour. © 2015 Society of Chemical Industry
- Subjects
MELON quality; FOOD aroma; VOLATILE organic compounds; GAS chromatography/Mass spectrometry (GC-MS); LEAST squares
- Publication
Journal of the Science of Food & Agriculture, 2016, Vol 96, Issue 7, p2352
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.7350