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- Title
Structure analysis and antimutagenic activity of a novel salt-soluble polysaccharide from Auricularia polytricha.
- Authors
Zhou, Jie; Chen, Yun; Xin, Mengting; Luo, Qinyao; Gu, Jialu; Zhao, Mengtian; Xu, Xiaolan; Lu, Xuanqi; Song, Guanglei
- Abstract
Background Auricularia polytricha is known to be a highly nutritious foodstuff. We report here the purification, structure characterization and antimutagenic activity in vivo of a 0.9% NaCl solution-soluble polysaccharide ( SSP) from the mycelia of A. polytricha. Results Analysis by high-performance liquid chromatography coupled to a TSK-G5000PWXL column and gel filtration chromatography on Sephacryl S-400 HR indicated that SSP is homogeneous with an average molecular weight of about 9.30 × 105 Da. The structure of SSP was revealed by chemical methods, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Results indicated that SSP is a glucan consisting of a1,3-β-glucan, 1,6-α-glucan, 1,4-α-glucan and 1,3-α-glucan backbone with a single 1,6-α- d-glucopyranosyl side-branching unit on every nine residues, on average, along the main chain. Atomic force microscopy indicates the presence of macromolecular species in morphology and shows a clear association of prolate particle. Meanwhile, SSP was found to significantly preventing micronuclei in polychromatic erythrocytes and reticulocytes of mice ( P < 0.01). Conclusion These results indicate that polysaccharide SSP from A. polytricha exhibits antimutagenic activity against the in vivo DNA-damaging effect of the indirectly acting alkylating agent cyclophosphamide.© 2013 Society of Chemical Industry
- Subjects
AURICULARIA; POLYTRICHACEAE; ANTIMUTAGENS; POLYTRICHALES; AGONOMYCETALES; HIGH performance liquid chromatography
- Publication
Journal of the Science of Food & Agriculture, 2013, Vol 93, Issue 13, p3225
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.6161