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- Title
Insights about minority HIV-1 strains in transmitted drug resistance mutation dynamics and disease progression.
- Authors
Leda, Ana Rachel; Hunter, James; Oliveira, Ursula Castro; Azevedo, Inacio Junqueira; Sucupira, Maria Cecilia Araripe; Diaz, Ricardo Sobhie
- Abstract
<bold>Objectives: </bold>The presence of minority transmitted drug resistance mutations was assessed using ultra-deep sequencing and correlated with disease progression among recently HIV-1-infected individuals from Brazil.<bold>Methods: </bold>Samples at baseline during recent infection and 1 year after the establishment of the infection were analysed. Viral RNA and proviral DNA from 25 individuals were subjected to ultra-deep sequencing of the reverse transcriptase and protease regions of HIV-1.<bold>Results: </bold>Viral strains carrying transmitted drug resistance mutations were detected in 9 out of the 25 patients, for all major antiretroviral classes, ranging from one to five mutations per patient. Ultra-deep sequencing detected strains with frequencies as low as 1.6% and only strains with frequencies >20% were detected by population plasma sequencing (three patients). Transmitted drug resistance strains with frequencies <14.8% did not persist upon established infection. The presence of transmitted drug resistance mutations was negatively correlated with the viral load and with CD4+ T cell count decay.<bold>Conclusions: </bold>Transmitted drug resistance mutations representing small percentages of the viral population do not persist during infection because they are negatively selected in the first year after HIV-1 seroconversion.
- Subjects
ANTIRETROVIRAL agents; SEXUALLY transmitted diseases; HIV-positive persons; HIV infections; GENETIC mutation
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2018, Vol 73, Issue 7, p1930
- ISSN
0305-7453
- Publication type
journal article
- DOI
10.1093/jac/dky132