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- Title
Brain-Derived Neurotrophic Factor Inhibits Peptidoglycan-Induced Inflammatory Cytokine Expression in Human Dental Pulp Cells.
- Authors
Takeda, Katsuhiro; Tokunaga, Naoko; Aida, Yusuke; Kajiya, Mikihito; Ouhara, Kazuhisa; Sasaki, Shinya; Mizuno, Noriyoshi; Fujita, Tsuyoshi; Kurihara, Hidemi
- Abstract
The aim of this study was to examine the anti-inflammatory effect of brain-derived neurotrophic factor (BDNF) on human dental pulp cells (HDPCs) and identify the intracellular signaling pathway involved. We investigated the effect of BDNF (50 ng/ml) on interleukin (IL)-6 and IL-8 expression in peptidoglycan (PGN)-treated HDPCs. An inhibition assay was performed with MAPK or NF-κB inhibitors to determine the possible signaling pathway. IL-6 and IL-8 mRNA, IL-6 and IL-8 protein, and phosphorylated p38 kinase activity were determined using real-time PCR, ELISA, and Western blot analysis, respectively. BDNF significantly attenuated PGN-induced IL-6 and IL-8 mRNA and protein levels in HDPCs. A p38 inhibitor also inhibited IL-6 and IL-8 mRNA transcription. PGN stimulated phosphorylated p38 kinase activity in HDPCs, which was inhibited by BDNF. Suppression of phosphorylated p38 kinase activity by BDNF in HDPCs inhibited increased IL-6 and IL-8 expression induced by PGN. Our findings suggest that BDNF regulates intracellular signaling molecule activities to exert its anti-inflammatory effect.
- Subjects
BRAIN-derived neurotrophic factor; PEPTIDOGLYCANS; DENTAL pulp; INTERLEUKIN-6; ENZYME-linked immunosorbent assay
- Publication
Inflammation, 2017, Vol 40, Issue 1, p240
- ISSN
0360-3997
- Publication type
Article
- DOI
10.1007/s10753-016-0474-4