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- Title
Structural basis of calcium and galactose recognition by the lectin PA-IL of Pseudomonas aeruginosa
- Authors
Cioci, Gianluca; Mitchell, Edward P.; Gautier, Catherine; Wimmerová, Michaela; Sudakevitz, Dvora; Pérez, Serge; Gilboa-Garber, Nechama; Imberty, Anne
- Abstract
The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 A˚ resolution, and the native protein was solved at 2.4 A˚ resolution. Each monomer adopts a β-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the co-ordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent of that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.
- Subjects
PSEUDOMONAS aeruginosa; LECTINS; GALACTOSE; PROTEINS
- Publication
FEBS Letters, 2003, Vol 555, Issue 2, p297
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01249-3